Analysis of mutants overexpressing PfdUTPase and HsdUTPase. a Immunofluorescence analysis of 3D7 wild-type cells (upper panels) and mutants overexpressing PfdUTPase (middle panels) and HsdUTPase (lower panels). A monoclonal antibody for PfdUTPase and a polyclonal antibody for HsdUTPase were used. Images were obtained using a confocal Leica TCS SP5 microscope and show a single optical plane/ Indirect immunofluorescence allowed for quantification of the intracellular distribution of the enzyme. In trophozoites the signal can be associated mostly with the cytosol although a certain overlap occurs with both mitochondria and nuclei. Intracellular localization of dUTPase in mutants overexpressing PfdUTPase was also analysed and appeared to be similar to what was observed in the parental cell line.
Pérez-Moreno G, Sánchez-Carrasco P, Ruiz-Pérez LM, Johansson NG, Müller S, Baragaña B, Hampton SE, Gilbert IH, Kaiser M, Sarkar S, Pandurangan T, Kumar V, González-Pacanowska D. Validation of Plasmodium falciparum dUTPase as the target of 5'-tritylated deoxyuridine analogues with anti-malarial activity. Malar J. 2019 18(1):392.