IFA with anti-PfGEXP10 antibody on early and mid-stage gametocytes. Fixed, permeabilized 3D7 gametocytes were simultaneously incubated with the serum against the PfGEXP10 protein or a control anti-GST antiserum (red fluorescence) and a serum against the gametocyte PVM marker PfMdv-1/peg3 (green fluorescence). Nucleus-specific Hoechst staining is shown on the right. 1 and 5, stage I; 2, stage II; 3 and 6, stage III; 4, late stage IV. Bar, 5 mm. Unlike the anti-GST control antiserum, the anti-PfGEXP10 antiserum reacted with stage I and mid-stage gametocytes and clearlyshowed a punctate fluorescence pattern beyond the PV compartment.

Silvestrini F, Lasonder E, Olivieri A, Camarda G, van Schaijk B, Sanchez M, Younis Younis S, Sauerwein R, Alano P. Protein export marks the early phase of gametocytogenesis of the human malaria parasite Plasmodium falciparum. Mol Cell Proteomics. 2010 Jul;9(7):1437-48.