PF3D7_1012900 autophagy-related protein 18

PfATG18 participates in autophagy-like pathway in P. falciparum. Colocalisation of PfATG18 with C. PfATG8 and E. PfATG5 upon starvation for indicated time period. Immunofluorescence staining of parasites using anti-PfATG18 (1:400), anti-PfATG8 (1:600) or anti-PfATG5 (1:400) antibodies labelled using Zenon antibody labelling system. Enlarged panel: magnified view of the dotted region shown in the Merge panel. Nucleus was stained with Hoechst. The Pearson’s coefficient (R) represents degree of colocalization between PfATG18 and PfATG8 evaluated from the signal within the square region shown in the shown in the enlarged panel, N=10 cells, n=3 experiments, Scale bar=5 μm. The number of PfATG18 puncta showing colocalisation with PfATG8 (C) and PfATG5 (E) was found to increase up to ~2-folds upon starvation. Taken together, the increase in PfATG18 expression at the mRNA as well as at the protein level and its colocalisation with PfATG8 and PfATG5 vesicles under starvation suggests that PfATG18 has a role in the autophagy-like pathway in this parasite.

Agrawal P, Manjithaya R, Surolia N. Autophagy-related protein PfATG18 participates in food vacuole dynamics and autophagy-like pathway in Plasmodium falciparum. Mol Microbiol. 2019 Dec 21

Other associated proteins

PFID Formal Annotation
PF3D7_1019900 autophagy-related protein 8
PF3D7_1430400 conserved Plasmodium protein, unknown function