MG132 treatment induces vacuole fission in PfATG18-GFP transfected parasites. A. Immunofluorescence assay using anti-falcipain-2 antibodies showing FV morphology in wild-type parasites treated with 400 nM MG132 for 4 h. Arrows indicate fragmented FV. C. Time-lapse confocal microscopy images of PfATG18-GFP labelled FV in MG132 treated transgenic P. falciparum 3D7 cells showing FV fission. White arrows indicate fragmented FV and blue arrows indicate enrichment of PfATG18-GFP at the contact site of multiple vacuoles upon fission. Images were acquired every 2 min for 30 min. N=10 cells, n=3 experiments, Scale bar=5 μm.
Agrawal P, Manjithaya R, Surolia N. Autophagy-related protein PfATG18 participates in food vacuole dynamics and autophagy-like pathway in Plasmodium falciparum. Mol Microbiol. 2019 Dec 21
Other associated proteins
|PF3D7_1012900||autophagy-related protein 18|