PfATG18 localisation is not affected in doxycycline-treated and PfATG8 knockdown

parasites. A. Immunofluorescence staining of parasites showing colocalisation of PfATG18 and PfATG8 in doxycycline-treated parasites upon 2 h starvation. Nucleus was stained with Hoechst. Enlarged panel: magnified view of the dotted region shown in the Merge panel. The Pearson’s coefficient (R) represents degree of colocalisation between PfATG18 and PfATG8 evaluated from the signal within the square region shown in the enlarged panel, N=10 cells, n=3 experiments, Scale bar=5 μm. C. PfATG5 and E. FP2 using anti-PfATG18 (1:400), anti-PfATG5 (1:400) and anti-falcipain-2 antibodies respectively using Zenon antibody labelling system in PfATG8 depleted (-aTC) and IPP rescued parasites upon 2 h starvation. Enlarged panel: magnified view of the dotted region shown in the Merge panel. The Pearson’s coefficient (R) represents the degree of colocalisation between PfATG18 and PfATG5 or FP2 evaluated from the signal within the square region shown in the enlarged panel, N=10 cells, n=3 experiments, Scale bar=5 μm.

Agrawal P, Manjithaya R, Surolia N. Autophagy-related protein PfATG18 participates in food vacuole dynamics and autophagy-like pathway in Plasmodium falciparum. Mol Microbiol. 2019 Dec 21