PGP is required for normal growth of P. falciparum asexual stages, and Δpgp mutant parasites selectively accumulate metabolites in the PPP and two nonstandard metabolites. Fluorescence microscopy of live PGP-GFP-infected RBCs. Infected RBCs were labeled with DAPI and visualized by differential interference contrast (DIC) and fluorescence microscopy. GFP fluorescence was present throughout the cytoplasm. PGP-green fluorescent protein (GFP) fusion protein is exclusively located in the cytoplasm of asexual blood stages.

Dumont L, Richardson MB, van der Peet P, Marapana DS, Triglia T, Dixon MWA, Cowman AF, Williams SJ, Tilley L, McConville MJ, Cobbold SA. The Metabolite Repair Enzyme Phosphoglycolate Phosphatase Regulates Central Carbon Metabolism and Fosmidomycin Sensitivity in Plasmodium falciparum. MBio. 2019 Dec 10;10(6). pii: e02060-19. PMID: 31822583.