PF3D7_1323500 PEXEL protease plasmepsin V

(A) PMV expression in asexual rings (R), asexual trophozoites (Tr), and gametocytes at stage I (day 2), stage II (day 4), stage III (day 6), stage IV (day 9), and stage V (day 12) by immunoblot with rabbit anti-PMV antibodies. Aldolase was a loading control. Giemsa-stained gametocyte stages are shown. Scale bar, 5 mm. (B) Immunofluorescence microscopy with mouse anti-PMV and rabbit anti-GAP45 antibodies show expression at the perinuclear ER in stage II gametocytes. The ER including PMV is redistributed around the cell, and GAP45 is incorporated into the IMC in stage III-IV gametocytes. Scale bar, 5 mm. (C) Schematic of GEXP05 and GEXP10 proteins used in this study. PEXEL is in red. HA, hemagglutinin tag; SS, signal sequence; TMD, transmembrane domain. (D) GEXP05-GFP and GEXP10-GFP are not expressed in asexual parasites, but they are expressed and exported in stage I gametocytes. Rabbit anti-PMV antibodies show expression at the perinuclear ER in asexual and sexual stages. Scale bar, 5 mm.

Jennison C, Lucantoni L, O'Neill MT, McConville R, Erickson SM, Cowman AF, Sleebs BE, Avery VM, Boddey JA. Inhibition of Plasmepsin V Activity Blocks Plasmodium falciparum Gametocytogenesis and Transmission to Mosquitoes. Cell Rep. 2019 29(12):3796-3806.e4.

Other associated proteins

PFID Formal Annotation
PF3D7_0113900 Plasmodium exported protein (hyp8)
PF3D7_0936600 Plasmodium exported protein (PHISTc), unknown function gametocyte exported protein 5
PF3D7_1222700 glideosome-associated protein 45
PF3D7_1471100 exported protein 2