Visualizing topologies of RIFIN and STEVOR proteins in mammalian cells. (B) The fluorescence pattern was examined by microscopy for both RIFIN (left panel) and STEVOR proteins (right panel), for both WT and mutants. (C) A positive (only gGFP) and negative control surfactant protein C (SP-CgGFP) for the fluorescence (left panel), and the corresponding western blot on the cell lysate for SP-C-gGFP. DAPI staining, GFP, and an overlay (composite) are indicated for the fluorescence images (Scale bars: 50 mm). The data depicted are representative of data obtained from three independent experiments.
Andersson A, Kudva R, Magoulopoulou A, Lejarre Q, Lara P, Xu P, Goel S, Pissi J, Ru X, Hessa T, Wahlgren M, von Heijne G, Nilsson I, Tellgren-Roth Å. Membrane integration and topology of RIFIN and STEVOR proteins of the Plasmodium falciparum parasite. FEBS J. 2019 Dec 10.
Other associated proteins