PF3D7_1343700 kelch protein K13

Kelch13 complex locates with AP-2m but is distinct to clathrin complex. Fluorescence microscopy images of parasites endogenously expressing 2xFKBP-GFP–tagged clathrin heavy chain (CHC) or AP-2m with episomal mCherry-Kelch13. Arrows mark overlap, and arrowheads mark similar region of Kelch13 foci. Scale bar, 5 mm. Merge, merged green and red channel; DAPI (nuclei). To test whether the Kelch13-Eps15 complex marks an endocytic structure, we colocalized Kelch13 with endogenously GFP-tagged clathrin heavy chain (CHC) or AP-2m). AP-2m–GFP foci colocalized with (arrowhead) or were found in the same region as Kelch13 foci (arrow), indicating an endocytosis role for the Kelch13-Eps15 compartment. Unexpectedly, CHC was present in foci that did not overlap with Kelch13. This showed that Kelch13 colocalizes with a compartment that contains AP-2 but is devoid of clathrin.

Birnbaum J, Scharf S, Schmidt S, Jonscher E, Hoeijmakers WAM, Flemming S, Toenhake CG, Schmitt M, Sabitzki R, Bergmann B, Fröhlke U, Mesén-Ramírez P, Blancke Soares A, Herrmann H, Bártfai R, Spielmann T. A Kelch13-defined endocytosis pathway mediates artemisinin resistance in malaria parasites. Science. 2020 Jan 3;367(6473):51-59.

Other associated proteins

PFID Formal Annotation
PF3D7_1218300 AP-2 complex subunit mu
PF3D7_1219100 clathrin heavy chain, putative