Histone acetylation and hyper-acetylation following treatment of gametocytes with TSA. (A,B) Presence of acetylated histones in gametocytes. Acetylated histones were detected in the different gametocyte stages (GC stage II-V) via immunolabeling using rabbit anti-H3K9ac (A) and anti-H4Kac4 (B) antibodies (green). Gametocytes were highlighted with mouse antibodies against the gametocyte marker Pfs230 (red). Nuclei were highlighted by Hoechst nuclear stain 33342 (blue). Bar, 5 μm. Histone acetylation was detected throughout the nuclei of gametocyte stages II-V highlighted by Hoechst staining and immunolabeling of Pfs230, respectively.
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