Electron micrographs of IEs enriched for binding to (A) CHOICAM-1 at 37 °C (IT4ICAM-1CHOICAM-137°) and 40 °C (IT4ICAM-1CHOOICAM-140°), and to (B) CHOCD36 at 37 °C (IT4CD36CHOCD3637°) and 40 °C (IT4CD36CHOOCD3640°). Number of knobby IEs relative to total number of analyzed IEs is indicated. In accordance with the results of IE enrichment to CSA at 40 °C, enrichment of IEs on ICAM-1 led to selection of knobby Ies (IT4ICAM-1CHOICAM-140°). Knobs were detected in 47% (n = 235) of the IT4ICAM-1CHOICAM-140° population (upper panel). On the other hand, enrichment of IEs to CD36 at 40 °C did not lead to selection of knobby IEs (B). These results could also be confirmed by measuring expression levels of kahrp and pfemp3. Only in the IT4ICAM-1CHOICAM-140° population, and not in IT4CD36CHOCD3640°, was a significant increase in expression (241-fold for kahrp and 56-fold for pfemp3)

Dörpinghaus M, Fürstenwerth F, Roth LK, Bouws P, Rakotonirinalalao M, Jordan V, Sauer M, Rehn T, Pansegrau E, Höhn K, Mesén-Ramírez P, Bachmann A, Lorenzen S, Roeder T, Metwally NG, Bruchhaus I. Stringent Selection of Knobby Plasmodium falciparum-Infected Erythrocytes during Cytoadhesion at Febrile Temperature. Microorganisms. 2020 Jan 25;8(2). pii: E174.

ˆ