Detection of PfPATs (=DHHCs) by immunofluorescence assay in P. falciparum blood stage schizonts. Mouse antisera raised against unique peptides derived from PfPATs -1, -2, -3 and -7 were used for immunostaining of slides smeared with P. falciparum schizont stages. Pre-immune sera were used as negative control (not shown). Nuclei were stained with DAPI (blue). The scale bar represents 5μm. IFA shows that PfDHHC PAT -1, -2, -3 and -7 are expressed in schizonts with minimal staining observed at ring and trophozoite stages. Apical staining was observed for PfDHHC PAT -1, -2, -3 and 7 in daughter merozoites. Colocalisation studies with microneme protein PfAMA1 indicates these PfPATs are localized at the apical end of the merozoites. However, PfPATs do not overlap completely with PfAMA1.
Siddiqui M, Singh S, Malhotra P, Chitnis C. Protein S-palmitoylation is responsive to external signals and plays a regulatory role in microneme secretion in Plasmodium falciparum merozoites. ACS Infect Dis. 2020 Jan 31.
Other associated proteins