Nuclear localization of candidate CAPs (chromatin-associated proteins). (c) Subcellular localization of the SMC3 protein during the asexual life stages of the parasite. (d) Subcellular localization of the CRWN-like protein during the asexual life stages of the parasite. (e) Immuno-fluorescence analysis showing the localization of SMC3 at the periphery of the parasite nucleus away from the heterochromatin regions marked by H3K9me3. (f) Colocalization of CRWN-like proteins to the heterochromatin regions of the parasite nucleus marked by H3K9me3. Bars indicate 2 μm. A single SMC3 focus is seen at the ring, trophozoite and schizont stages (c). This CRWN-like protein localizes to a single focus inside the nucleus at ring and schizont stages (d). At the ring and schizont stages, the SMC3 proteins localized to the nuclear periphery away from the heterochromatin regions marked by H3K9me3 (e). However, co-localization of H3K9me3 and CRWN-like proteins was observed (>90 % of ring-stage parasites), the localization of CRWN-like proteins appeared to extend to regions adjacent to heterochromatin regions of the nucleus as well (f).

Batugedara G, Lu XM, Saraf A, Sardiu ME, Cort A, Abel S, Prudhomme J, Washburn MP, Florens L, Bunnik EM, Le Roch KG. The chromatin bound proteome of the human malaria parasite. Microb Genom. 2020 Feb 4.