Indirect immunofluorescence images of schizont stage KC5 wildtype and C3h-KO parasites probed with anti-CLAG3 antibody (green). Scale bar, 5 μm. We successfully generated the C3h-KO limiting dilution clone that lacks an intact clag3 gene. Immunoblotting and immunofluorescence imaging confirmed complete absence of CLAG3 protein in this parasite (C).
Gupta A, Bokhari AAB, Pillai AD, Crater AK, Gezelle J, Saggu G, Nasamu AS,
Ganesan SM, Niles JC, Desai SA. Complex nutrient channel phenotypes despite Mendelian inheritance in a Plasmodium falciparum genetic cross. PLoS Pathog. 2020 16(2):e1008363.