Left panel: Clemastine but not HSF1A interferes with microtubule biogenesis during P. falciparum mitosis. Representative confocal z-sections of P. falciparum infected erythrocytes after treatment with 10 μM clemastine, 10 μM HSF1A, or 0.1% DMSO at 32 hpi for 12 h (n > 30). Cells were stained with Hoechst (blue) and anti–α-tubulin antibody (red) to visualize parasite nuclei and microtubule structures, respectively. Data are representative of two to three biological replicates. Clemastine causes aberrant microtubule morphology and α-tubulin reduction in P. falciparum. (right pannels). Representative confocal z-sections of P. falciparum-infected erythrocytes after treatment with 10 μM clemastine or 10 μM HSF1A at 30 hpi for 12 h (upper right) or for 20 h (lower rightB). Cells were stained with Hoechst (blue) and anti–α-tubulin antibody (red) to visualize parasite nuclei and microtubule structures, respectively. Clemastine influences tubulin at various time points during schizogony. Data are representative of two to three biological replicates.

Lu KY, Quan B, Sylvester K, Srivastava T, Fitzgerald MC, Derbyshire ER. Plasmodium chaperonin TRiC/CCT identified as a target of the antihistamine clemastine using parallel chemoproteomic strategy . Proc Natl Acad Sci U S A. 2020;201913525.