PfERC is not required for protein trafficking or organellar biogenesis. Left panel: Representative SIM images of PfERC-glmS (PfERC tagged with the inducible version of the ribozyme gene, glmSn) and PfERC-M9 (inactive version of the ribozyme, M9) schizonts incubated with GlcN for 48 h and stained with anti-MSP1 and the nuclear stain DAPI. From top to bottom, the images are DAPI (blue), anti-MSP1 (green), and fluorescence merge. Bar, 2 mm. Right panel Representative SIM images of PfERC-glmS and PfERC-M9 schizonts incubated with GlcN for 48 h and

stained with anti-RAP1 and the nuclear stain DAPI. From top to bottom, the images represent DAPI (blue), anti-RAP1 (green), and fluorescence merge. Bar, 2 mm. data show that there were no differences between developing PfERC-glmS and PfERC-M9 merozoites with respect to trafficking of MSP1 to the surface after knockdown.

Fierro MA, Asady B, Brooks CF, Cobb DW, Villegas A, Moreno SNJ, Muralidharan V. An Endoplasmic Reticulum CREC Family Protein Regulates the Egress Proteolytic Cascade in Malaria Parasites. mBio. 2020 Feb 25;11(1). pii: e03078-19.