AP-2m-KO severely disrupts schizont maturation. (A) Antibodies against the ER (PMV), Golgi apparatus (ERD2), PVM (EXP2), PPM (MSP1), IMC (GAP45, GAP50), apicoplast (CDC48), micronemes (AMA1), rhoptries (RON4), episomal K13 (GFP), and AP-2m (HA) were used to stain 3D7-AP-2m-floxed-3xHA schizonts with rap treatment (KO) or without (wt). All organelle markers have been false colored green regardless of fluorophore-conjugated secondary antibody used for clarity. Nuclei have been false colored blue. IMC-TM, transmembrane component of inner membrane complex. Scale bar, 2 mm.
Henrici RC, Edwards RL, Zoltner M, van Schalkwyk DA, Hart MN, Mohring F, Moon RW, Nofal SD, Patel A, Flueck C, Baker DA, Odom John AR, Field MC, Sutherland CJ. The Plasmodium falciparum Artemisinin Susceptibility-Associated AP-2 Adaptin μ Subunit is Clathrin Independent and Essential for Schizont Maturation. mBio. 2020 Feb 25;11(1). pii: e02918-19.
Other associated proteins
|PF3D7_0711000||AAA family ATPase, CDC48 subfamily|
|PF3D7_0918000||glideosome-associated protein 50 secreted acid phosphatase|
|PF3D7_1116000||rhoptry neck protein 4|
|PF3D7_1133400||apical membrane antigen 1|
|PF3D7_1218300||AP-2 complex subunit mu|
|PF3D7_1222700||glideosome-associated protein 45|
|PF3D7_1323500||PEXEL protease plasmepsin V|
|PF3D7_1343700||kelch protein K13|
|PF3D7_1353600||ER lumen protein retaining receptor|
|PF3D7_1471100||exported protein 2|