Localization of AP-2μ-2xFKBPP-GFP and ER-marker PDI by immunoelectron microscopy. Electron micrograph of typical vesicular colocalization of AP-2μ-2xFKBP-GFP (18 nm gold particles) and PDI (12 nm gold particles) in developing trophozoites. N, nucleus; ER, endoplasmic reticulum; FV, food vacuole; H, hemozoin; black arrow, AP-2μ at the plasma membrane; empty arrow, AP-2μ in vesicles; white-outlined arrows, AP-2μ in the cytosol; white arrows, AP-2μ at the ER. At least some cytosolic AP-2 μ-positive vesicles also contained Rab5B, an effector of endosome-like transport between the plasma membrane and the FV. Parasites expressing AP-2μ -2xFKBP-GFP showed a similar localization
and distribution by immuno-EM.
Henrici RC, Edwards RL, Zoltner M, van Schalkwyk DA, Hart MN, Mohring F, Moon RW, Nofal SD, Patel A, Flueck C, Baker DA, Odom John AR, Field MC, Sutherland CJ. The Plasmodium falciparum Artemisinin Susceptibility-Associated AP-2 Adaptin μ Subunit is Clathrin Independent and Essential for Schizont Maturation. mBio. 2020 Feb 25;11(1). pii: e02918-19.
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_1134100 | protein disulfide isomerase, putative |