PF3D7_1410400 rhoptry-associated protein 1

PfCERLI1HAGlmS knockdown alters rhoptry antigen distribution. a 3D reconstructions of super-resolution (Airyscan) micrographs for PfCERLI1HAGlmS schizont cultures that were either left untreated or treated with glucosamine (+2.5mM GLCN) before being stained with DAPI (nucleus), anti-HA (PfCERLI1), anti-RAP1 (rhoptry bulb) and anti-RON4 (rhoptry neck) antibodies. RAP1 signal that overlapped with RON4 after knockdown of PfCERLI1 was significantly reduced. These data indicate that PfCERLI1 knockdown causes changes in the distribution of antigens within the rhoptry, most notably for RAP1, and increases the spatial segregation between rhoptry neck (RON4) and bulb (RAP1) markers.

Liffner B, Frölich S, Heinemann GK, Liu B, Ralph SA, Dixon MWA, Gilberger TW, Wilson DW. PfCERLI1 is a conserved rhoptry associated protein essential for Plasmodium falciparum merozoite invasion of erythrocytes. Nat Commun. 2020 11(1):1411.

Other associated proteins

PFID Formal Annotation
PF3D7_0210600 cytosolically exposed rhoptry leaflet interacting protein, protein CERLI1
PF3D7_1116000 rhoptry neck protein 4
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