Expression and localization of PfmtRPS12, PfmtRPS17 and PfmtRPS18. PfmtRPS12-3HA, PfmtRPS17-3HA, or PfmtRPS18-3HA was localized to the parasite mitochondrion. A co-staining of anti-HA antibody and MitoTracker is shown. Note that the tubular mitochondrion in trophozoites often becomes artificially “fragmented” after fixation with 4% paraformaldehyde/0.0075% glutaraldehyde. Data shown are representatives of two independent IFA experiments. At least n = 200 parasites were examined in each IFA experiment/ all three localized to the mitochondrion.

Ling L, Mulaka M, Munro J, Dass S, Mather MW, Riscoe MK, Llinás M, Zhou J, Ke H. Genetic ablation of the mitoribosome in the malaria parasite Plasmodium falciparum sensitizes it to antimalarials that target mitochondrial functions. J Biol Chem. 2020 Apr 9:jbc.RA120.012646. PMID: 32273345.