K13 shows an increased association with the mitochondria post DHA pulse. (B) Representative fluorescence microscopy images and 3D reconstructions of NF54WT-attBGFP-K13WT (left) or NF54WTattB-3HA-K13C580Y (right) ring-stage parasites co-stained with MitoTracker Deep Red and anti-GFP antibodies. (E) Representative fluorescence microscopy images and 3D reconstructions of Cam3.IIR539T ring-stage parasites 12h post DHA pulse or DMSO mock treatment. Scale bars: 1 μm. (F) Representative IEM images of NF54WTattB-3HA-K13C580Y trophozoites treated with DHA (9 nM for 3h) or a DMSO vehicle control and stained with anti-HA antibodies to detect K13. Arrowhead highlights location of interest. M, mitochondria; N, nucleus. Scale bar: 100 nm. (G) 3D volume reconstruction of untreated late Cam3.IIR539T and Cam3.IIWT trophozoites triply stained with MitoTracker, anti-BIP (ER, green) and anti-K13 E3 (purple).
Gnädig NF, Stokes BH, Edwards RL, Kalantarov GF, Heimsch KC, Kuderjavy M, Crane A, Lee MCS, Straimer J, Becker K, Trakht IN, Odom John AR, Mok S, Fidock DA. Insights into the intracellular localization, protein associations and artemisinin resistance properties of Plasmodium falciparum K13. PLoS Pathog. 2020 Apr 20;16(4):e1008482.
Other associated proteins