Localization of PfMAAP in schizonts and merozoites. (A) Co-localization of anti-PfMAAP peptide antibody reactivities (green) with the apical markers PfAMA1 (microneme, red), PfRAP2 (rhoptry bulb, red) PfRON4 (rhoptry neck, red). Staining shows clear punctate apical staining for all three apical markers; (B) anti-PfMAAP (red) co-localization with inner membrane complex (IMC) protein GAP45 and the merozoite surface markers PfMSP1 (green). (C) The localization of anti-PfMAAP antibody reactivities (green) in free merozoites relative to PfAMA1, PfRAP2, and PfRON4. The staining patterns observed showed a clear merozoite surface staining pattern in developing schizonts and a predominantly apical staining in free merozoites and on those within mature rupturing schizonts (A). The staining pattern was compared by co-localization with relative to PfAMA1 (micronemes), PfRAP2 (rhoptry bulb), and PfRON4 (rhoptry neck). Co-localization with a-AMA1 antibodies (a micronemal marker), showed apical staining of merozoites within the mature schizont (A) and on free merozoites (B), with a small proportion showing diffuse surface staining of the merozoite by the a-PfMAAP antibody (A, top panel). Punctate apical staining was also observed with co-localization of the a-PfMAAP with the a-PfRAP2 (a marker for the rhoptry bulb) and a-PfRON4 (a rhoptry neck marker).
Aniweh Y, Nyarko PB, Charles-Chess E, Ansah F, Osier FHA, Quansah E, Thiam LG, Kamuyu G, Marsh K, Conway DJ, Tetteh KKA, Awandare GA. Plasmodium falciparum Merozoite Associated Armadillo Protein (PfMAAP) Is Apically Localized in Free Merozoites and Antibodies Are Associated With Reduced Risk of Malaria. Front Immunol. 2020 Apr 7;11:505
Other associated proteins
|PF3D7_0501600||rhoptry-associated protein 2|
|PF3D7_1035900||merozoites-associated armadillo repeats protein|
|PF3D7_1116000||rhoptry neck protein 4|
|PF3D7_1133400||apical membrane antigen 1|