Upper panel: Kelch13 compartment proteins are involved in endocytosis of host cell cytosol. (A) Correlative light and electron microscopy with a cell expressing endogenously tagged Eps15-GFP: confocal image (scale bar, 2 mm), EM section and magnification thereof (scale bars, 0.5 mm). Arrowheads indicate vesicles containing material of host cell density. N, nucleus; FV, food vacuole. Lower panel: Kelch13 complex locates with AP-2m but is distinct to clathrin complex. Fluorescence microscopy images of parasites endogenously expressing 2xFKBP-GFP–tagged clathrin heavy chain (CHC) or AP-2m with episomal mCherry-Kelch13. Arrows mark overlap, and arrowheads mark similar region of Kelch13 foci. Scale bar, 5 mm. Merge, merged green and red channel; DAPI (nuclei).

Birnbaum J, Scharf S, Schmidt S, Jonscher E, Hoeijmakers WAM, Flemming S, Toenhake CG, Schmitt M, Sabitzki R, Bergmann B, Fröhlke U, Mesén-Ramírez P, Blancke Soares A, Herrmann H, Bártfai R, Spielmann T. A Kelch13-defined endocytosis pathway mediates artemisinin resistance in malaria parasites. Science. 2020 367(6473):51-59. PMID: 31896710