MTRAP Is Expressed in Sexual Stages of P. falciparum (A) Fluorescence microscopy with anti-PfMTRAP (green), anti-Pfg377 (red), and DAPI (blue) in wildtype P. falciparum sexual stages matured in vitro. Stages III, IV, and V gametocytes are shown. BF, brightfield. Scale bar, 5 mm. MTRAP was strongly detected in the sexual stages. When synchronous P. falciparum gametocytes at stages III, IV, or V of maturation were analyzed by immunofluorescence assay, MTRAP was detected predominantly in stage V gametocytes and only weakly in stages.(B) Fluorescence microscopy with anti-PfMTRAP (green), anti-Band3 (red), and DAPI (blue) in wildtype P. falciparum sexual stages matured in vitro. A gametocyte nonactivated (preactivation), a gametocyte activated for 30 s in vitro, and an egressed gamete after 600 s of activation in vitro are shown. BF, brightfield. Scale bar, 5 mm. The pattern of MTRAP staining was thus followed over time during P. falciparum gametocyte activation. Prior to activation, crescent-shaped gametocytes, displaying peripheral MTRAP staining, were surrounded by an intact EM labeled with anti-Band3 antibody.
Bargieri DY, Thiberge S, Tay CL, Carey AF, Rantz A, Hischen F, Lorthiois A, Straschil U, Singh P, Singh S, Triglia T, Tsuboi T, Cowman A, Chitnis C, Alano P, Baum J, Pradel G, Lavazec C, Ménard R. Plasmodium Merozoite TRAP Family Protein Is Essential for Vacuole Membrane Disruption and Gamete Egress from Erythrocytes. Cell Host Microbe. 2016 20(5):618-630.
Other associated proteins
|PF3D7_1250100||osmiophilic body protein g377|