PF3D7_0104300 ubiquitin carboxyl-terminal hydrolase 1, putative

Kelch13 location and interactome. (Left panel) Fluorescence microscopy images showing parasites with endogenously GFP-tagged Kelch13 (K13) (17) with coexpressed STVR-SDEL (ER), Sec13p (ER exit sites), P40 (phosphatidylinositol 3-phosphate sensor: food vacuole), ACP (apicoplast), or endogenously GFP-tagged mutated Kelch13 (K13C580Y) with episomal mCherry-Kelch13wt.

(Right panel) Fluorescence microscopy in parasites with endogenously GFP-tagged DiQ-BioID hits (KIC1-10 or short name) colocalized with episomal mCherry-Kelch13. Arrowheads indicate Kelch13 foci overlapping with the KIC signal. Scale bars, 5 mm. Merge, merged green and red channel; DAPI, 4′,6-diamidino-2-phenylindole (nuclei).

Birnbaum J, Scharf S, Schmidt S, Jonscher E, Hoeijmakers WAM, Flemming S, Toenhake CG, Schmitt M, Sabitzki R, Bergmann B, Fröhlke U, Mesén-Ramírez P, Blancke Soares A, Herrmann H, Bártfai R, Spielmann T. A Kelch13-defined endocytosis pathway mediates artemisinin resistance in malaria parasites. Science. 2020 367(6473):51-59.

Other associated proteins

PFID Formal Annotation
PF3D7_0606000 kelch13 interaction candidate kic1
PF3D7_0609600 probable protein, unknown function
PF3D7_0813000 kelch13 interaction candidate kic7
PF3D7_0914400 kelch13 interaction candidate kic3
PF3D7_0915400 ATP-dependent 6-phosphofructokinase
PF3D7_1014900 kelch13 interaction candidate kic8
PF3D7_1017300 golgi re-assembly stacking protein 2
PF3D7_1122500 kelch13 interaction candidate kic10
PF3D7_1138700 kelch13 interaction candidate kic5
PF3D7_1227700 kelch13 interaction candidate kic2
PF3D7_1230700 protein transport protein SEC13
PF3D7_1246300 kelch13 interaction candidate kic4
PF3D7_1322000 nucleoside-diphosphatase, putative
PF3D7_1323000 beta-hydroxyacyl-ACP dehydratase
PF3D7_1343700 kelch protein K13
PF3D7_1442400 kelch13 interaction candidate kic9
PF3D7_1459100 GTP-binding protein, putative