Kelch13 location and interactome. (Left panel) Fluorescence microscopy images showing parasites with endogenously GFP-tagged Kelch13 (K13) (17) with coexpressed STVR-SDEL (ER), Sec13p (ER exit sites), P40 (phosphatidylinositol 3-phosphate sensor: food vacuole), ACP (apicoplast), or endogenously GFP-tagged mutated Kelch13 (K13C580Y) with episomal mCherry-Kelch13wt.
(Right panel) Fluorescence microscopy in parasites with endogenously GFP-tagged DiQ-BioID hits (KIC1-10 or short name) colocalized with episomal mCherry-Kelch13. Arrowheads indicate Kelch13 foci overlapping with the KIC signal. Scale bars, 5 mm. Merge, merged green and red channel; DAPI, 4′,6-diamidino-2-phenylindole (nuclei).
Birnbaum J, Scharf S, Schmidt S, Jonscher E, Hoeijmakers WAM, Flemming S, Toenhake CG, Schmitt M, Sabitzki R, Bergmann B, Fröhlke U, Mesén-Ramírez P, Blancke Soares A, Herrmann H, Bártfai R, Spielmann T. A Kelch13-defined endocytosis pathway mediates artemisinin resistance in malaria parasites. Science. 2020 367(6473):51-59.
Other associated proteins
|PF3D7_0606000||kelch13 interaction candidate kic1|
|PF3D7_0609600||probable protein, unknown function|
|PF3D7_0813000||kelch13 interaction candidate kic7|
|PF3D7_0914400||kelch13 interaction candidate kic3|
|PF3D7_1014900||kelch13 interaction candidate kic8|
|PF3D7_1017300||golgi re-assembly stacking protein 2|
|PF3D7_1122500||kelch13 interaction candidate kic10|
|PF3D7_1138700||kelch13 interaction candidate kic5|
|PF3D7_1227700||kelch13 interaction candidate kic2|
|PF3D7_1230700||protein transport protein SEC13|
|PF3D7_1246300||kelch13 interaction candidate kic4|
|PF3D7_1343700||kelch protein K13|
|PF3D7_1442400||kelch13 interaction candidate kic9|
|PF3D7_1459100||GTP-binding protein, putative|