Co-IFA of PfRrp6 and PfRrp4 or PfRrp6 and PfRNase II with the PfRrp6-Ty1::HA-PfRrp4 or PfRrp6-Ty1::PfRNase II-HA line, respectively. DAPI, 4=,6-diamidino-2-phenylindole. (G, left) The bar graph represents the percentage of colocalization or noncolocalization of PfRrp6-PfRrp4 or PfRrp6-PfRNase II, respectively. For each experiment, ~50 nuclei were examined. Error bars represent standard errors of the means (SEM) for three biological replicates. ***, P < 0.001 (C2 test). (G, right) A schematic illustration of the subcellular distribution of RNA exosome-associated ribonucleases (Rrp6 and Dis3) and PfRNase II.
Fan Y, Shen S, Wei G, Tang J, Zhao Y, Wang F, He X, Guo G, Shang X, Yu X, Ma Z, He X, Liu M, Zhu Q, Le Z, Wei G, Cao J, Jiang C, Zhang Q. Rrp6 Regulates Heterochromatic Gene Silencing via ncRNA RUF6 Decay in Malaria Parasites. mBio. 2020 11(3):e01110-20.
Other associated proteins
|PF3D7_0410400||exosome complex component RRP4, putative|