Processed product of RH1 does not interact with AMA1 in late schizont stage parasites. (a-c) Processed product of PfRH1 interacts with each other by Proximity Ligation Assay (PLA). PLA assay was performed using plus and minus probe conjugated to PfRH1 mAbs against EBD, MD and RVIII, respectively. Dapi (blue) was used as nuclear stain. Bright field images and merged images with bright field images are shown. The interaction between EBD or MD or RVIII was observed as individual red dot in schizonts. (d-f) The PLA between AMA1 and EBD or MD or RVIII in schizonts was shown. Dapi (blue) was used as nuclear stain. Bright field images and merged images with bright field images are shown. No interaction was

observed as individual red dot in schizonts.

Gunalan K, Gao X, Yap SSL, Lai SK, Ravasio A, Ganesan S, Li HY, Preiser PR. A processing product of the Plasmodium falciparum reticulocyte binding protein RH1 shows a close association with AMA1 during junction formation. Cell Microbiol. 2020 May 25:e13232. PMID: 32452132