PF3D7_0903700 alpha tubulin 1

Distribution of male gamete types 25 min after activation. Male gametes were classified into four categories after IFA with anti–-tubulin (stains microtubules, including the flagella) and anti–glycophorin A (an erythrocyte membrane marker) antibodies and 4′,6-diamidino-2-phenylindole (DAPI) staining of nuclei. Replication defect: Parasites that rounded up but had not replicated the genome (single nucleus). Delayed egress: Parasites that replicated the genome and

formed axonemes but were still inside the red blood cells. Others: Other defects such as parasites lacking properly formed axonemes. Exflagellating: Parasites that developed correctly. Data are presented as the average and SEM of three or four independent experiments (E5ind, 389 male gametes; 1.2Bind, 769 male gametes; NF54, 406 male gametes). (D) Representative images of the categories described in (C). Scale bars, 10 mm.

Llorà-Batlle O, Michel-Todó L, Witmer K, Toda H, Fernández-Becerra C, Baum J, Cortés A. Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum. Sci Adv. 2020 6(24):eaaz5057. PMID: 32577509