Localization of ISN1 (IMP-specific 5'-nucleotidase, putative) in Plasmodium. a Indirect immunofluorescence microscopy with anti-PfISN1 antibody on intraerythrocytic asexual (3D7 strain) and sexual (3D7A strain) stages of P. falciparum. The absence of signal from pre-immune control ruled out non-specific binding (not shown). The experiment was repeated at least three times. Live-cell fluorescence imaging of episomally expressed PfISN1-GFP fusion protein in 3D7_PfISN1-GFP (b) and PbANKA_PfISN1-GFP (c) parasites. Imaging of PbANKA_PfISN1-GFP was performed at least three times with parasites collected from independent mice, and imaging of 3D7_PfISN1-GFP was performed at least three times with parasites harvested from independent cultures. Scalebars correspond to 1 μm.
Carrique L, Ballut L, Shukla A, Varma N, Ravi R, Violot S, Srinivasan B, Ganeshappa UT, Kulkarni S, Balaram H, Aghajari N. Structure and catalytic regulation of Plasmodium falciparum IMP specific nucleotidase. Nat Commun. 2020 11(1):3228.