Localization of ISN1 (IMP-specific 5'-nucleotidase, putative) in Plasmodium. a Indirect immunofluorescence microscopy with anti-PfISN1 antibody on intraerythrocytic asexual (3D7 strain) and sexual (3D7A strain) stages of P. falciparum. The absence of signal from pre-immune control ruled out non-specific binding (not shown). The experiment was repeated at least three times. Live-cell fluorescence imaging of episomally expressed PfISN1-GFP fusion protein in 3D7_PfISN1-GFP (b) and PbANKA_PfISN1-GFP (c) parasites. Imaging of PbANKA_PfISN1-GFP was performed at least three times with parasites collected from independent mice, and imaging of 3D7_PfISN1-GFP was performed at least three times with parasites harvested from independent cultures. Scalebars correspond to 1 μm.

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