Upper panel: Immunofluorescence analysis (IFA) of paraformaldehyde-fixed gametocyte infected erythroblast at 6 dpi stained with anti-Pf11-1 antibodies (green) and with anti-glycophorin A (GPA) labelling the erythroblast membrane. the gametocyte-specific protein Pf11-1 was detected both in the parasite and in the infected erythroblast. Lower panel: Infection with the Hsp70-GFP line (upper panels) and with the Pfs16-GFP line (lower panels) were observed by fluorescent microscopy at 2 dpi (upper panels and lower left panel) and 6 dpi (lower right panel). Erythroblast membrane is stained with PKH-26 (red). Hsp70-GFP line expressing GFP throughout the entire parasite life cycle under the control of the constitutive promoter hsp70 to monitor total parasitemia, and the Pfs16-GFP line expressing GFP under the control of the gametocyte-specific promoter Pfs16 to monitor gametocytemia.
Neveu G, Richard C, Dupuy F, Behera PK, Volpe F, Subramani PA, Marcel-Zerrougui B, Vallin P, Andrieu M, Minz AM, Azar N, Martins RM, Lorthiois A, Gazeau F, Lopez-Rubio JJ, Mazier D, Silva AKA, Satpathi S, Wassmer S, Verdier F, Lavazec C. Plasmodium falciparum sexual parasites develop in human erythroblasts and affect erythropoiesis. Blood. 2020 blood.2019004746.
Other associated proteins
|PF3D7_0406200||parasitophorous vacuole membrane protein s16; sexual stage-specific protein s16|