Effect of compound 5(R) on the development of P. falciparum. A tightly synchronized culture of P. falciparum at ring stage was treated or not

with 2 μM 5(R). (B) Immunofluorescence assay on infected erythrocytes at time 45 h, at segmented schizont stage. Formation of merozoites membrane

was visualized by labeling the internal membrane complex (α-mTIP) and the membrane surface (α-MSP1). The labeling of nuclei with Hoechst

showed that all merozoites, in control and treated parasites, possessed a nucleus. daughter cells developed normally in the presence of compound 5(R) and that the DNA content of the newly formed merozoites seemed unaffected by the treatment.

Cheviet T, Wein S, Bourchenin G, Lagacherie M, Périgaud C, Cerdan R, Peyrottes S. β-Hydroxy- and β-Aminophosphonate Acyclonucleosides as Potent

Inhibitors of Plasmodium falciparum Growth. J Med Chem. 2020 Jul 20. doi: 10.1021/acs.jmedchem.0c00131.