The nuclear localization of PfSR1 is adjacent to nuclear pore complexes (NPCs). IFA of NF54 early stage parasites co-transfected with PfSR1myc) and PfSec13-GFP using anti-myc (PfSR1, red) and anti-GFP (PfSec13, green) antibodies. Nuclei were stained DAPI (blue). Upper panel: Punctuated nuclear distribution of PfSR1myc in early ring stage shows strict association with the NPC. Lower panel: diffused nuclear distribution of PfSR1myc in late ring stage is

adjacent to the NPC. By co-transfecting PfSR1-myc and PfSec13-GFP, an NPC marker, into NF54 parasites, we could localize both proteins by IFA. In ring stage parasites, when PfSR1 is nuclear and the NPC cluster is found at a distinct spot at the nuclear periphery, PfSR1-myc and PfSec13 were found to localize to the same region in approximately 80% of the nuclei that show a distinct punctate distribution of PfSR1-myc

Eshar S, Altenhofen L, Rabner A, Ross P, Fastman Y, Mandel-Gutfreund Y, Karni R, Llinás M, Dzikowski R. PfSR1 controls alternative splicing and steady-state RNA levels in Plasmodium falciparum through preferential recognition of specific RNA motifs. Mol Microbiol. 2015 Jun;96(6):1283-97. .