Strains generated to study the function and localization of DegP2. a, b The DegP2 coding sequence was replaced with YFP to generate the ΔDegP2 line, which was subsequently complemented with an HA-tagged copy of DegP2 (dark green) under the regulation of the TUB1 promoter (a). Separately, the endogenous locus of DegP2 was first tagged with the Ty epitope, and a point mutation was introduced at the catalytic serine (b). DegP2-HA, DegP2-Ty, and DegP2S569A-Ty co-localized with the mitochondrial marker mtHSP70. Merged image additionally displays YFP (green) for ΔDegP2 strains and DNA stain (blue) for the HA-stained samples. Scale bar is 5 μm.
Harding CR, Sidik SM, Petrova B, Gnädig NF, Okombo J, Herneisen AL, Ward KE, Markus BM, Boydston EA, Fidock DA, Lourido S. Genetic screens reveal a central role for heme metabolism in artemisinin susceptibility. Nat Commun. 2020 11(1):4813..
Other associated proteins