PfFis1 localization with N- or C-terminal tagging and PfFis1 KD without tags. The pLN-based construct used for ectopic expression of N-terminal 3HA-tagged PfFis1. The CTS of PfFis1 (KSFKYF) is highlighted in red. Representative IFA images show localization of 3HA-PfFis1. Parasites were probed with mouse anti-HA (green), MitoTracker (red), and DAPI (4’,6-diamidino-2-phenylindole) (blue) and merged with differential interference contrast (DIC). Scale bar, 2 mm. Western blotting data show the expression of 3HA-PfFis1. Anti-HA and anti-PfEXP2 (loading control) antibodies were used. (B) The pLN-based construct used for ectopic expression of C-terminal 3Myc-tagged PfFis1. Representative IFA images show localization of PfFis1-3Myc. Parasites were probed with mouse anti-Myc (green), MitoTracker (red), and DAPI (blue) and merged with DIC. Scale bar, 2 mm. Western blotting data show the expression of PfFis1-3Myc. Anti-Myc and anti-PfEXP2 (loading control) antibodies were used.

Maruthi M, Ling L, Zhou J, Ke H. Dispensable Role of Mitochondrial Fission Protein 1 (Fis1) in the Erythrocytic Development of. Plasmodium falciparum mSphere. 2020 5(5):e00579-20.