AMR4 is an imported apicoplast protein and does not share a postprenylation processing pathway with PfICMT. (A and B) Representative immunofluorescence images showing localization of AMR4-3xHA (A) and PfICMT-3xHA. AMR4 colocalizes with the apicoplast marker ACP and is present on the organelle’s distinctive branching structures during schizogony (B) compared to the apicoplast marker ACP after 3 days of either no treatment or treatment with 10 mM actinonin/200 mM IPP (+Actinonin +IPP). Bars, 5 mm. PfICMT does not colocalize with ACP, and its localization is unaffected by actinonin treatment (B), indicating that it does not localize to the apicoplast.
Meister TR, Tang Y, Pulkoski-Gross MJ, Yeh E. CaaX-Like Protease of Cyanobacterial Origin Is Required for Complex Plastid Biogenesis in Malaria Parasites. mBio. 2020 11(5):e01492-20.
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0913500 | apicoplast-minus ipp-rescued 4 |
PF3D7_1236800 | protein-s-isoprenylcysteine o-methyltransferase |