Immunofluorescence detection of the P. falciparum PVM-resident antigens RAP1 and EXP2 after haemolysis. a Immunofluorescence staining of P. falciparum after haemolysis with saponin, representative images of > 200 parasites. b Immunofluorescence staining of P. falciparum after haemolysis with streptolysin O, representative images of n = 70 parasites. Note, only RAP1 was detected, indicating that the EXP2 epitope faces the PV lumen. In the merged images, EXP2 and RAP1 signals are shown in green; Hoechst signal is shown in blue. Scale bar, 5 μm
Quadt KA, Smyrnakou X, Frischknecht F, Böse G, Ganter M. Plasmodium falciparum parasites exit the infected erythrocyte after haemolysis with saponin and streptolysin O. Parasitol Res. 2020 Oct 22.
Other associated proteins
|PF3D7_1410400||rhoptry-associated protein 1|