PfMCMBP depletion causes abnormal nuclear morphology with additional MTOC ectopic foci and formation of extended mitotic spindle microtubules. (A) Representative pictures from [+]/[−] Shld1 PfMCMBP3HADD parasites (40-44 h.p.i.) after PFA-fixation and probing with mouse anti-CrCen1 (1:500, green) and mouse anti-αTubulin (1:200, red) followed by isotype-specific goat anti-mouse antibodies. Three different z-slices from a single representative [+] Shld1 parasite are shown in rows i-iii. Panels iv-vi represent three different PfMCMBP-deficient parasites cultured [−] Shld1. Images were taken with LSM880 Airyscan using the 63X Objective. Yellow arrows indicate bipolar spindle and yellow asterisk indicate short microtubule between duplicated MTOC in WT condition. Scale bar at 1 μm.

Absalon S, Dvorin JD. Depletion of the Mini-Chromosome Maintenance Complex Binding Protein allows the progression of cytokinesis despite abnormal karyokinesis during the asexual development of Plasmodium falciparum. Cell Microbiol. 2020 Oct 30:e13284.