PF3D7_0208500 acyl carrier protein

Upper panel" Epifluorescence images of D10 parasites treated with DOX and metals. Magnet plus sorbitol-synchronized parasites were treated as rings with 10 μM DOX ±500 μM FeCl3 or CaCl2 and imaged 36 hours later (green = ACPL-GFP, blue = nuclearHoechst stain); Lower panel: Fluorescence microscopy of live, synchronized D10 parasites untreated or treated with 15 μM DFO and imaged 36 hours later (green = ACPL-GFP, blue = nuclear Hoechst stain). 20-30 total parasites were examined for each treatment condition on each given day for duplicate experiments, and data were plotted as the average percentage of parasites in each population that displayed an elongated, punctate, or dispersed apicoplast GFP signal.

Okada M, Guo P, Nalder SA, Sigala PA. Doxycycline has distinct apicoplast-

specific mechanisms of antimalarial activity. Elife. 2020 Nov 2;9:e60246

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