10 μM doxycycline kills P. falciparum with first-cycle, apicoplast-specific activity, Epifluorescence images of synchronized parasites treated as rings with 10 μM DOX ±200 μM IPP and imaged 36 or 65 449 hours later (green = ACPL-GFP, blue = nuclear Hoechst stain) . Fluorescence microscopy of live, synchronized D10 parasites untreated or treated with 15 μM DFO and imaged 36 hours later (green = ACPL-GFP, blue = nuclear Hoechst stain).

Okada M, Guo P, Nalder SA, Sigala PA. Doxycycline has distinct apicoplast-

specific mechanisms of antimalarial activity. Elife. 2020 Nov 2;9:e60246.. PMID: 33135634