PF3D7_0930300 merozoite surface protein 1

In vivo expression and co-localization assay. Expression analysis and co-localization of PfPFD-6 with MSP-1 at different asexual stages of parasite life cycle. Smears of methanol-fixed Pf3D7-infected erythrocytes were stained with anti-PfPFD-6 antibodies (1:250) and anti-MSP-1 antibodies (1:250), followed by incubation with Alexa Fluorconjugated secondary antibodies (Alexa Fluor 488, red; Alexa Fluor 546, green). DIC: differential interference contrast image, DAPI: nuclear staining 40, 6-diamidino-2-phenylindole (blue); MSP-1: mouse anti-MSP-1 (green); PfPFD-6: anti-PfPFD-6 antibody (red); merge: overlay of PfPFD-6 proteins with MSP-1. Scale bar represents 2 μm. Immunofluorescence assays (IFAs) were performed to visualize expression of PfPFD-6 and co-localization of PfPFD-6 with MSP-1 during the asexual stages of Pf using protein-specific antibodies on cultured parasites. Expression of PfPFD-6 and MSP-1 begins at mid trophozoite stage of parasite’s life cycle. At schizont stages, these seem to remain confined within the parasitophorous vacuole (PV) of Pf. PfPFD-6 showed negligible co-localization with MSP-1 at the trophozoite stage of parasite development. In the schizont stage, significant overlapping of signals was observed within the PVM, suggestive of their coexistence.

Kumar V, Rumaisha, Behl A, Munjal A, Abid M, Singh S. Prefoldin subunit 6 of Plasmodium falciparum binds merozoite surface protein-1 (MSP-1). FEBS Open Bio. 2020 Nov 3.

Other associated proteins

PFID Formal Annotation
PF3D7_0512000 prefoldin subunit 6