Co-localization of PF3D7_1372300 and ATS (acidic terminal domain of var PF3D7_0800200) using indirect immunofluorescence. Smears of methanol-fixed Pf3D7-infected erythrocytes were incubated with anti-PF3D7_1372300 rabbit IgG (1:500) and anti-ATS rat IgG (1:1,000), followed by incubation with Alexa Fluor-conjugated secondary antibody (Alexa Fluor 488, rabbit; Alexa Fluor 594, rat). IgG from healthy rabbits and rats were used as negative controls, and buffer without antibody served as a blank control. Parasite nuclei were stained with DAPI. The co-localization results showed that the two proteins have fluorescence overlap at the erythrocyte membrane during the trophozoite and schizont stages.

Yang B, Wang X, Jiang N, Sang X, Feng Y, Chen R, Wang X, Chen Q. Interaction Analysis of a Plasmodium falciparum PHISTa-like Protein and PMID: 33281807