3D7morc-glmS construct strategy and localization. We engineered 3D7 parasites by incorporating a glmS ribozyme within the 3′-untranslated region of the pfmorc gene along with an additional triple hemagglutinin tag before the ribozyme. This parasite line was referred to as 3D7morc-glmS immunofluorescence assay and confocal microscopy to localize PfMORC in P. falciparum-infected erythrocytes (Ring, Trophozoite and Schizont Stage). Co-localization with nucleus stain DAPI confirms the GFP imaging that PfMORC localizes in the nucleus.
Singh MK, Tessarin-Almeida G, Dias BKM, Pereira PS, Costa F, Przyborski JM, Garcia CRS. A nuclear protein, PfMORC confers melatonin dependent synchrony of the human malaria parasite P. falciparum in the asexual stage. Sci Rep. 2021 11(1):2057.