Generation of DiCre-inducible PfHP1-PbHP1 hybrid mutants. Representative live-cell fluorescence images showing the localization of GFP-tagged PfHP1-PbHP1 hybrid proteins in 3D7/HP1-hyb-PbHinge and 3D7/HP1-hyb-PbCSD parasites in late schizonts (LS) (40 to 48 hpi; generation 1 [Gen1], 40 h after RAP treatment) and in late-ring-stage progeny (LR) (16 to 24 hpi, generation 2). Nuclei were stained with Hoechst dye. DIC, differential interference contrast. Scale bar, 5mm. Both PfHP1-hyb-PbHinge-GFP and PfHP1-hyb-PbCSD-GFP localized to perinuclear heterochromatin foci in schizonts and in the ring-stage progeny in a pattern indistinguishable from that observed for wild-type PfHP1-GFP. Thus, replacement of the PfHP1 hinge domain or CSD with those from PbHP1 retains proper heterochromatin localization of HP1 in P. falciparum.

Bui HTN, Passecker A, Brancucci NMB, Voss TS. Investigation of Heterochromatin Protein 1 Function in the Malaria Parasite Plasmodium falciparum Using a Conditional Domain Deletion and Swapping Approach. mSphere. 2021 6(1):e01220-20..