Association of PfRab5b and PfArf1 GTPases rather than PfRab1b in adjacent to the nucleus. Transformant parasites carrying the PfArf1-RFP (A, red) or PfRab1b-RFP (B, red) constructs with PfRab5bQ94L-YFP-DD (green) under the dual Pfef1a promoter were stabilized with Shld1, and were then used in the immunofluorescence assay. The fluorescence of RFP and YFP was captured. Arrowheads indicate the colocalization of PfArf1 and PfRab1b with PfRab5bQ94L. The nuclei were stained with DAPI (blue). Representative images showing mononuclear early trophozoite (upper), two nuclear late trophozoite (middle), and multinucleated early schizont (lower) stages are shown. White arrowheads indicate the colocalization of PfRab5bQ94L-YFP-DD and PfArf1-RFP or PfRab1b-RFP. The bars indicate 2 μm. In the mononuclear early trophozoite stage, 2 or 3 nuclear late trophozoite, and multinucleated early schizont stages, the RFP signals of PfArf1-RFP and RFP-PfRab1b were observed as juxtanuclear punctate structures, and these colocalized with the YFP fluorescence from PfRab5bQ94L-YFPDD.
Taku I, Hirai T, Makiuchi T, Shinzawa N, Iwanaga S, Annoura T, Nagamune K, Nozaki T, Saito-Nakano Y. Rab5b-Associated Arf1 GTPase Regulates Export of N-Myristoylated Adenylate Kinase 2 From the Endoplasmic Reticulum in Plasmodium falciparum. Front Cell Infect Microbiol. 2021 10:610200.
Other associated proteins
|PF3D7_1020900||ADP-ribosylation factor 1|
|PF3D7_1310600||ras-related protein Rab-5B secretory complex protein 61 alpha|