PF3D7_0512600 ras-related protein Rab-1B

PfArf1 and PfRab1b regulated the export of PEXEL-positive Rifin to the erythrocyte cytoplasm. Parasites expressing Rifin-RFP and PfArf1-YFP-DD

(A) or DD-YFP-PfRab1b (C) were examined via the immunofluorescence assay. The export of Rifin was also decreased by the expression of inactive mutants of PfArf1, but not the active GTPfixed mutant and the wild-type PfArf1, which cycles GTP- and GDP-bound states (A). This result suggests that the GTP-bound state of PfArf1, but not GDP-fixed mutant, is necessary for the export of Rifin, or for the correct activity of PfRab1b. Fluorescence signals from RFP (red), YFP (green), and DAPI (blue) are shown. Wild-type PfArf1 or PfRab1b (upper panels), active mutant PfArf1Q71L or PfRab1bQ67L (middle panels), and the inactive mutant PfArf1T31N or PfRab1bS22N (lower panels) are shown. White dotted lines indicate the parasite plasma membrane. The arrowheads indicate dot-like exported Rifin-RFP signals. The bars indicate 2 μm. PfRab1b was involved in the transport of the PEXEL-positive export protein, Rifin, and the expression of the inactive PfRab1b mutant showed decreased activity of Rifin export to erythrocyte cytosol.

Taku I, Hirai T, Makiuchi T, Shinzawa N, Iwanaga S, Annoura T, Nagamune K, Nozaki T, Saito-Nakano Y. Rab5b-Associated Arf1 GTPase Regulates Export of N-Myristoylated Adenylate Kinase 2 From the Endoplasmic Reticulum in Plasmodium falciparum. Front Cell Infect Microbiol. 2021 10:610200. PMID: 33604307

Other associated proteins

PFID Formal Annotation
PF3D7_1020900 ADP-ribosylation factor 1