PfJ2 is an essential, ER-resident protein. A) PfJ2apt parasites were fixed and stained with DAPI (blue) and with antibodies against HA (green) and the ER marker PfPMV (red). Ring (R), Trophozoite (T), and Schizont (S) stage parasites are shown. Z-stack Images were deconvoluted and shown as a single, maximum intensity projection. Scale bar represents 5 μm. Co-localization between PfJ2 and the ER-marker Plasmepsin V (PfPMV) revealed that PfJ2 is in fact an ER-resident protein, consistent with the localization of episomally over expressed PfJ2 previously reported.
Cobb DW, Kudyba HM, Villegas A, Hoopmann MR, Baptista RP, Bruton B, Krakowiak M, Moritz RL, Muralidharan V. A redox-active crosslinker reveals an essential and inhibitable oxidative folding network in the endoplasmic reticulum of malaria parasites. PLoS Pathog. 2021 7(2):e1009293.
Other associated proteins
|PF3D7_1108700||heat shock protein 40, type ii|