For immunofluorescent analysis, Pf-3D7 schizonts were stained with Pf-mAbs (green, first column) and costained with anti-hsp70 antibody (purple, second column) and the nuclear stain DAPI (blue, third column). Images are representative of at least two independent experiments. Scale bar, 5 μm.The specificity of Pf mAbs was confirmed by IFA by using Pf blood-stage parasites. The PfAMA1-specific mAb B10 had a micronemal staining pattern consistent with the cellular location of PfAMA1, while PfMSP1-specific mAbs stained the merozoite surface as expected.
Hopp CS, Sekar P, Diouf A, Miura K, Boswell K, Skinner J, Tipton CM, Peterson ME, Chambers MJ, Andrews S, Lu J, Tan J, Li S, Doumbo S, Kayentao K, Ongoiba A, Traore B, Portugal S, Sun PD, Long C, Koup RA, Long EO, McDermott AB, Crompton PD. Plasmodium falciparum-specific IgM B cells dominate in children, expand with malaria, and produce functional IgM. J Exp Med. 2021 218(4):e20200901
Other associated proteins
|PF3D7_0930300||merozoite surface protein 1|
|PF3D7_1133400||apical membrane antigen 1|