SEA1-null parasites complete DNA replication but display unusual nuclear morphology. (A) IFA showing localization of SEA1-HA3 in proximity to a-tubulin and centrin throughout the development of SEA1-HA:loxP trophozoites/early schizonts. SEA1-HA3 is absent in RAP-treated parasites. Scale bar, 10mm. (B) IFA showing localization of SEA1-HA3 in proximity to a-tubulin and centrin in SEA1-HA:loxP schizonts (;45 h postinvasion, hpi). Scale bar, 10mm. Double-staining analysis using superresolution imaging showed that the SEA1-HA3 signal comprised a single focus within each nucleus that was often flanked by twinned signals corresponding to components of the nuclear division apparatus. The signals for centrin, a key component of the centrosome, flanked the SEA1-HA3 foci more distally. We interpreted these results as strongly indicative of a subcellular localization of SEA1 in the
dividing nucleus at a position that could be consistent with the previously proposed
Perrin AJ, Bisson C, Faull PA, Renshaw MJ, Lees RA, Fleck RA, Saibil HR, Snijders AP, Baker DA, Blackman MJ. Malaria Parasite Schizont Egress Antigen-1 Plays an Essential Role in Nuclear Segregation during Schizogony. mBio. 2021 12(2):e03377-20.
Other associated proteins
|PF3D7_0903700||alpha tubulin 1|