Immunolocalization of SEA1 to a nuclear location. (A) IFA showing localization and relative intensity of SEA1-HA3 signals in trophozoite (top)-, schizont (middle)-, and merozoite (bottom)-stage parasites. Scale bar, 10mm. DIC, differential interference contrast. (B) IFA showing localization of SEA1-HA3 and AMA1 in schizonts. The HA signal is strongest in less mature schizonts (bottom right) than in very mature segmented schizonts that express the late-stage marker protein AMA1 (top left). Scale bar, 10mm. (C) Superresolution immunofluorescence images showing localization of SEA1-HA3 in proximity to other proteins at the nuclear periphery. SEA1-HA3 loci are closely flanked by sometimes twinned a-tubulin signals (left) and, slightly further away, by often twinned signals for the centrosome marker centrin (right). Scale bars, 5mm.

Perrin AJ, Bisson C, Faull PA, Renshaw MJ, Lees RA, Fleck RA, Saibil HR, Snijders AP, Baker DA, Blackman MJ. Malaria Parasite Schizont Egress Antigen-1 Plays an Essential Role in Nuclear Segregation during Schizogony. mBio. 2021 12(2):e03377-20.