Mixed-stage transfected parasites were incubated with the pH probe, LysoSensor Blue. Shown are bright field, LysoSensor Blue, GFP and merged GFP/LysoSensor Blue images. The LysoSensor Blue fluorescence is largely associated with the DV, while the GFP chimaera is also present in cytostomal vesicles (arrows) that do not stain with the LysoSensor Blue. Scale bar, 2 μm.
Klonis N, Tan O, Jackson K, Goldberg D, Klemba M, Tilley L. Evaluation of pH during cytostomal endocytosis and vacuolar catabolism of haemoglobin in Plasmodium falciparum. Biochem J. 2007 407(3):343-54.